Roderick H.J. de Hilster


PhD: ongoing 

Master: university of Groningen – Biomedical sciences research master
Bachelor: university of Groningen – biomedical sciences




3D-bioprinted airways: A new breath to replace animal models for lung disease research

This research project focuses on generating a novel model of the human airway wall using 3D bioprinting. To this end we develop bioinks/hydrogels derived from nondiseased and diseased human lung extracellular matrix (ECM). By developing this model we hope to reduce the need for animal experiments in lung research and a better platform in discovering and testing new treatments. Furthermore, with this model we hope to answer some questions on the involvement of the ECM in the pathophysiology of chronic lung diseases such as COPD.


Keywords: 3D, 3D (bio)printing, Extra Cellular Matrix

Migulina, N, de Hilster, RHJ, Bartel, S, Vedder, RHJ, van den Berge, M, Nagelkerke, A et al.. 3-D culture of human lung fibroblasts decreases proliferative and increases extracellular matrix remodeling genes. Am J Physiol Cell Physiol. 2024;326 (1):C177-C193. doi: 10.1152/ajpcell.00374.2023. PubMed PMID:37955339 .

Nizamoglu, M, de Hilster, RHJ, Zhao, F, Sharma, PK, Borghuis, T, Harmsen, MC et al.. An in vitro model of fibrosis using crosslinked native extracellular matrix-derived hydrogels to modulate biomechanics without changing composition. Acta Biomater. 2022;147 :50-62. doi: 10.1016/j.actbio.2022.05.031. PubMed PMID:35605955 .

Martinez-Garcia, FD, de Hilster, RHJ, Sharma, PK, Borghuis, T, Hylkema, MN, Burgess, JK et al.. Architecture and Composition Dictate Viscoelastic Properties of Organ-Derived Extracellular Matrix Hydrogels. Polymers (Basel). 2021;13 (18):. doi: 10.3390/polym13183113. PubMed PMID:34578013 PubMed Central PMC8470996.

de Hilster, RHJ, Sharma, PK, Jonker, MR, White, ES, Gercama, EA, Roobeek, M et al.. Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue. Am J Physiol Lung Cell Mol Physiol. 2020;318 (4):L698-L704. doi: 10.1152/ajplung.00451.2019. PubMed PMID:32048864 PubMed Central PMC7191637.

Lépine, AFP, de Hilster, RHJ, Leemhuis, H, Oudhuis, L, Buwalda, PL, de Vos, P et al.. Higher Chain Length Distribution in Debranched Type-3 Resistant Starches (RS3) Increases TLR Signaling and Supports Dendritic Cell Cytokine Production. Mol Nutr Food Res. 2019;63 (2):e1801007. doi: 10.1002/mnfr.201801007. PubMed PMID:30412339 PubMed Central PMC6767581.

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